The Evaluation of antimicrobial efficacy of herbal extracts (Tridax procumbens, aegle marmelos) and 5% sodium hypochlorite as irrigants against Enterococcus Faecalis
an in vitro study
Keywords:
Biofilm, E. faecalis, herbal, NaOCl, root canal irrigant, Tridaxprocumbens, AegleMarmelosAbstract
Introduction: This study was done to evaluate the antimicrobial efficacy of Tridax procumbens, Aegle Marmelos and 5% sodium hypochlorite against E. faecalis biofilm formed on tooth substrate.
Methods: Extracted human teeth were biomechanically prepared, vertically sectioned, placed in the tissue culture wells exposing the root canal surface to E. faecalisto form a biofilm. At the end of the 3rd and 6th week all groups were treated for 10 minutes with the test solutions and control and were analysed qualitatively and quantitatively.
Results: Qualitative assay with 3-week biofilm showed complete inhibition of bacterial growth with Tridax procumbens, Aegle Marmelos and NaOCl, except saline, which showed presence of bacterial growth. In quantitative analysis, Aegle and saline-treated tooth samples showed 1,344 ±123 CFU/mL and 186.4 ₓ109 ± 4.1 ₓ109CFU/mL (mean and standard deviation), respectively. Qualitative assay with 6-week biofilm showed growth when treated with Tridax and marmelos whereas NaOCl has shown complete inhibition. All treated groups have shown a significant reduction of bacterial population compared with the control group, and NaOCl showed 100% reduction .
Conclusions: 5% sodium hypochlorite showed maximum antibacterial activity against E. Faecalis biofilm formed on toothsubstrate. Tridaxprocumbens, Aeglemarmelos also showed statistically significant antibacterial activity.
