Comparative Study of Frequency of Micronuclei Using Papanicolaou Stain in Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

Authors

  • shravani renukuntla post graduate student
  • Vaishali N 2. Assistant Professor, Oral and Maxillofacial Pathology, Government Dental College and Hospital, Hyderabad, Telangana.
  • Preethi P Post Graduate, Oral and Maxillofacial Pathology, Government Dental College and Hospital, Hyderabad, Telangana.
  • Shobhita KC Post Graduate, Oral and Maxillofacial Pathology, Government Dental College and Hospital, Hyderabad, Telangana.
  • Priyanka M Post Graduate, Oral and Maxillofacial Pathology, Government Dental College and Hospital, Hyderabad, Telangana.
  • Poornima R Post Graduate, Oral and Maxillofacial Pathology, Government Dental College and Hospital, Hyderabad, Telangana.

Keywords:

Lichen planus, Micronuclei, Oral Potentially Malignant Disorders, Oral squamous cell Carcinoma, Papanicolaou stain.

Abstract

Background: Early recognition is crucial for successful treatment and good prognosis of oral cancer. A simple, non-invasive and reasonably effective technique for initial evaluation of a suspicious oral lesion is exfoliative cytology. Micronuclei being structures presenting after genomic damage can be used as a screening tool in early detection of cancer.

Method: 60 subjects, 30 with potentially malignant disorders (Oral Lichenplanus and Oral Submucous Fibrosis), 15 with Oral Squamous Cell Carcinoma and 15 with apparently normal mucosa were included in the study. Cytological smears were collected from these groups and micronuclei were examined using Papanicolaou stain. A Oneway ANOVA was used to compare the mean micronuclei distribution among different study groups.                                              

Results: We observed an increase in frequency of micronuclei from normal to potentially malignant disorders to cancerous lesions. Analysis has shown significant differences between various study groups (P<0.05), with highest score observed in oral squamous cell carcinoma group, intermediary in potentially malignant disorders and least in controls.                                                                                                                 

Conclusion: The oral mucosal micronuclei can be used as a marker of epithelial carcinogenic progression.                                                                      

 

Published

2019-05-01

Issue

Vol. 10 No. 01 (2018): IJDA

Section

Original Research